Anti-T7-tag pAb-Agarose

Polyclonal Antibody conjugated to agarose of Gel: 200 µl targeting T7 for IP.

Target: T7

Product Type: Antibody

Application: IP

Clonality: Polyclonal

Conjugate: Agarose

Isotype: Rabbit Ig (aff.)

Immunogen: Binding synthetic peptide carrier protein (MASMTGGQQMGC)

Host Species: Rabbit

Formulation: 340 μg of anti-T7-tag polyclonal antibody covalently coupled to 200 μL of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 400 μL.

Research Area: Epitope Tag, Purification/Separation

Description/Background:

Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag
antibodies can serve as universal purification or detection reagents for any tag-containing protein. The T7-tag antibody is directed against the 11 amino acid of gene 10 leader peptide expressed by many translation vectors (MASMTGGQQMG). Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage. Anti-T7-tag antibodies are useful reagents to easily identify, detect, or purify T7-Tag fusion proteins from cell lysates.

Storage Temperature: 4°C

Protocols: IP

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.