Anti-Kaede pAb (Polyclonal Antibody)

Target: Kaede

Product Type: Antibody

Application: WB

Clonality: Polyclonal

Conjugate: Unlabeled

Isotype: IgG

Immunogen: Full-length Kaede

Host Species: Rabbit

Formulation: 100 µL volume of 10 mM phosphate buffer containing 0.3 M NaCl and 0.09 % NaN3, pH 8.0. *Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty of water when disposing materials containing azide into drain.

Research Area: Fluorescent Proteins

Description/Background: CoralHue® Kaede protein emits green fluorescence that can be converted to red. The red fluorescence is comparable in intensity to the green and is stable under usual aerobic conditions. The green-to-red conversion is highly sensitive to irradiation with UV or violet light (350-410 nm). Maximal illumination results in a 2,000-fold increase in the ratio of red-to-green signal. The excitation lights used to elicit red and green fluorescence do not induce the photoconversion. This property provides a simple and powerful technique for regional optical marking.

Storage Temperature: This antibody solution is stable for one year from the date of purchase when stored at -20°C.

Protocols: WB

Source: This antibody was fractioned by salting out and gel filtration on a Sephadex G-200 column. The rabbit was immunized with the recombinant CoralHue® Kaede.

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.