Coagulation Factor XII, Human, ELISA kit

The coagulation factor XII ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing coagulation factor XII. Biotinylated tracer antibody will bind to the captured. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the coagulation factor XII standards (log). The coagulation factor XII concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Target: coagulation factor XII

Product Type: ELISA Kit

Application: ELISA

Research Area: Cancer, Immunology

Description/Background:

The human coagulation factor XII ELISA kit is to be used for the in vitro quantitative determination of coagulation factor XII in plasma and serum.

Storage Temperature: Product should be stored at 2-8 °C. Under recommended storage conditions, product is stable for at least six months.

Protocols: ELISA

Sensitivity: 0.8 to 50 ng/ml

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.