Anti-His-tag mAb-Alexa Fluor® 594 (Monoclonal Antibody)

Monoclonal antibody conjugated to Alexa Fluor® 594 of 50 µl targeting His-tag for ICC, IH.

Product Type: Antibody

Application: ICC, IH

Clonality: Monoclonal

Conjugate: Alexa Fluor® 594

Isotype: IgG2a ĸ

Immunogen: 6xHis-tagged protein

Host Species: Mouse

Formulation: 50 µg of IgG in 50 µL volume of PBS containing 1% BSA and 0.1% ProClin150.

Research Area: Epitope Tag, Purification/Separation, Smart-IP

Description/Background: The His-tag (6xHis-tag) is one of the most common tags used to facilitate the purification of recombinant proteins. Metal chelate affinity chromatography is widely used for purification of His-tagged proteins.　This specific antibody is useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of the recombinant proteins.

Storage Temperature: This antibody solution is stable for one year from the date of purchase when stored at 4°C.

Protocols: ICC, IH

Clone Number: OGHis

Concentration: 1 mg/mL

Source: This antibody was purified from hybridoma (clone OGHis) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell SP-1 with Balb/c mouse splenocyte immunized with 6xHis tagged protein.

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.