Anti-Proton Pump (H, K-ATPaseβ subunit) (Mouse) mAb (Monoclonal Antibody)

Monoclonal antibody of 100 μg targeting Proton pump (H,K-ATPase β subunit) for IHC, IPP, WB.

Target: Proton pump

Product Type: Antibody

Application: IHC, IP, WB

Clonality: Monoclonal

Conjugate: Unlabeled

Isotype: IgG1

Immunogen: Hybridoma, fusion of spleen cells Balb / c nu removal of thymus cells and induce Sp2/0-Ag14 myeloma, autoimmune gastritis / + mouse neonatal

Host Species: Mouse

Species Reactivity: Human, Bovine, Mouse, Rat

Formulation: 100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Research Area: Apoptosis

Description/Background: Hydrogen-potassium adenosine triphosphatase (H+, K+-ATPase) belongs to a family of P-type cation-transporting ATPases that also includes Ca2+-ATPase and Na+, K+-ATPase. H+, K+ ATPase is responsible for acid secretion into the stomach and catalyzes electroneutral exchange of cytoplasmic hydrogen ions and external potassium ions coupled with ATP hydrolysis. The enzyme is found in gastric parietal cells where it is located in cytoplasmic vesicles or apical plasma membranes of the secretory canaliculus. H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic α subunit and a smaller but heavily glycosylated β subunit. The α subunit of H+, K+-ATPase has 10 transmembrane domains and contains sites for ATP binding and its acylphosphorylation, binding sites of proton pump inhibitors, and sites responsible for ion recognition.

Storage Temperature: -20°C

Protocols: IHC, IP, WB

Clone Number: 2B6

Concentration: 1 mg/ml

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.