Anti-Phospho-Syntide-2 mAb

Monoclonal Antibody of 100 μg targeting Syntide-2 for ELISA.

Target: Syntide-2

Product Type: Antibody

Application: ELISA

Clonality: Monoclonal

Conjugate: Unlabeled

Isotype: IgG2b

Immunogen: a synthetic phosphopeptide Syntide-2 (PLARTL(pS)VAGLPGKK; synthetic substrate for CaM-Kinase II)

Host Species: Mouse

Formulation: Supplied in 20 mM phosphate buffer (pH 7.5), 300 mM NaCl, 50 % glycerol.

Research Area: Cancer, Cell Biology, Drug Discovery

Description/Background: Syntide-2, a peptide based on phosphorylation site two of glycogen synthase, is an exogenous substrate designed for CaM-Kinase (calcium/calmodulin dependent kinases) in the serine-threonine kinase family. CaM-kinase II is a multifunctional calcium/calmodulin dependent protein kinase involved in neuronal functions. This anti-Phospho-Syntide-2 monoclonal antibody has been validated with CaM-kinase II, however it has the potential for use in evaluating other serine threonine kinases such as CaM-Kinase IIa, PKCµ, Akt1, Akt2, Akt3, and PKA. The relative Vmax/Km ratios of the known Ca2+-dependent protein kinases for syntide-2 were determined to be as follows: protein kinase II, 100; protein kinase C, 22; phosphorylase kinase, 2; myosin light chain kinase, 0.005.

Storage Temperature: -20°C

Protocols: ELISA

Clone Number: MS-6E6

Sensitivity: Anti-Phospho-Syntide-2 mAb (MS-6E6) detects phosphorylated Syntide-2 only when phosphorylated at serine residue, by ELISA.

Concentration: 1 mg/mL x 100 µL

Source: Monoclonal antibody is produced by immunizing mice with a synthetic phosphopeptide Syntide-2, PLARTL(pS)VAGLPGKK, which is synthetic substrate for CaM-Kinase II (Calmodulin dependent protein kinase II). IgG is purified by protein A-sepharose chromatography.

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.