NMNAT1(Human, Active)

NMNAT1(Human, Active)

Target: NMNAT1

Product Type: Recombinant Protein

Species Reactivity: Human

Formulation: Recombinant NMNAT1 is supplied frozen in a buffer containing 50 mM Potassium phosphate buffer, pH 7.4, 1 mM DTT, 1 mM MgCl2, 0.5 mM EDTA, 300 mM KCl and 50 % glycerol. Use a same buffer for dilution when needed.

Research Area: Immunology

Description/Background: Nicotinamide mononucleotide adenylyltransferase 1 (NMNAT1) (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, transferring the adenylyl moiety of ATP to nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) resulting in the formation of NAD or NaAD and the release of pyrophosphate. As this reaction is reversible, the enzyme may in principle be used to form ATP and NMN from NAD and pyrophosphate. This enzyme could be a potential target for therapeutical applications, because its activity is rather low in tumor cells. The deduced protein contains an N-terminal nuclear localization signal. Immunofluorescence microscopy localized endogenous NMNAT1 to the nucleus in human fibroblasts and in a hepatoma cell line. It was demonstrated that NMNAT1 inhibited recombinant human poly(ADP-ribose) polymerase-1 by about 35 %, and it completely prevented the formation of branched ADP-ribose polymers

Storage Temperature: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, AVOID REPEATED HANDLING AND MULTIPLE FREEZE/THAW CYCLES. Unopened vial at -70 °C, for 1 year after delivery.

Protocols:

Molecular Weight: 33 kDa

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.