HA tagged Protein PURIFICATION KIT

HA tagged protein purification kit is designed for the isolation of HA tagged protein from cell culture supernatants containing serum and cell lysate under neutral pH condition.

Target: HA

Product Type: Kit

Application: IP

Research Area: Epitope Tag, Purification/Separation, Smart-IP

Description/Background: <p>Recombinant tagged protein purification methods and kits are now widely recognized. The HA epitope tag, YPYDVPDYA, is derived from Human influenza hemagglutinin (HA) 98-106 a.a. This HA tag is also commonly used in such as mammalian cell expression vectors. MBL’s HA-tagged Protein PURIFICATION KIT is designed for the isolation of HA-tagged protein from cell culture supernatants containing serum and cell lysate under neutral pH condition. Severe conditions such as acidic or alkaline elution denature protein structure. However, a neutral pH elution can preserve protein activity and native conformation. MBL has developed the Anti-HA tag Beads to purify HA-tagged proteins quickly and efficiently. As the Beads can be used at neutral pH, the purified proteins can maintain the activity and conformation. The elution of HA-tagged proteins from the Beads is achieved by the addition of the HA peptide. As the HA peptide competes with HA-tagged proteins on the Beads, the purified proteins do not lose the protein activity. Using a Spin Column resulting in high efficiency has optimized the simple procedure of this kit.</p>

Protocols: IP

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.